Simultaneous determination of nine phenolic acids in Salvia miltiorrhiza by RRLC-UV.
- Author:
Li WANG
1
;
Mingbo ZHAO
;
Wenshun HE
;
Ke ZAN
;
Kaishun BI
;
Pengfei TU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; instrumentation; methods; Drugs, Chinese Herbal; analysis; Hydroxybenzoates; analysis; Salvia miltiorrhiza; chemistry; Spectrophotometry, Ultraviolet
- From: China Journal of Chinese Materia Medica 2009;34(19):2481-2484
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a RRLC-UV method for simultaneous determination of nine phenolic acids in Salvia miltiorrhiza.
METHOD3,4-Dihydroxyphenylacetic acid was used as internal standard, and analysis was carried out on an Agilent Zorbax Eclipse Plus C18 (2.1 mm x 50 mm, 1.8 microm) column with acetonitrile-0.1% formic acid aqueous solution as mobile phases in gradient elution. The flow rate was 0.3 mL x min(-1), and the UV detector was monitored at 286 nm.
RESULTAll calibration curves showed good linear regression within test ranges (r > or = 0.999 5); and the overall recoveries were in the range of 98.2%-102.6%, with RSD less than 3.1% (n = 3). The overall RSD of precision test were less than 2.4%.
CONCLUSIONThe developed method was simple, rapid, accurate and reproducible, and can be used for the quality control of S. miltiorrhiza.
