Distribution study of two constituents in rat after oral administration of Smilax china extract.
- Author:
Huilian HUANG
1
;
Guangtong CHEN
;
Zhiqiang LU
;
Jinqiang ZHANG
;
Dean GUO
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Oral; Animals; Chromatography, High Pressure Liquid; methods; Drug Evaluation, Preclinical; Drugs, Chinese Herbal; administration & dosage; pharmacokinetics; Female; Plant Extracts; administration & dosage; analysis; pharmacokinetics; Rats; Rats, Sprague-Dawley; Smilax; chemistry; Stilbenes; administration & dosage; analysis; pharmacokinetics
- From: China Journal of Chinese Materia Medica 2009;34(19):2512-2515
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the distribution in rat after oral administration of Smilax china extract.
METHODOxyresveratrol and resveratrol were quantitatively determined by HPLC/DAD method in rat tissues including heart, liver, spleen, lung and kidney after oral administration of S. china extract. The separation was performed on a Zorbax SB-C18 column (4.6 mm x 250 mm, 5 microm) with acetonitrile-water using linear gradient elution and UV detection at wavelength of 320 nm. The flow rate was 1.0 mL x min(-1) and the column was kept at 40 degrees C.
RESULTThe HPLC/DAD method for the simultaneous determination of oxyresveratrol and resveratrol in rat tissues was developed.
CONCLUSIONThe method has been successfully applied for the distribution study of two active constituents in rat through oral administration of S. china extract.
