Determination of pseudoprodioscin in Dioscorea panthaica by HPLC.
- Author:
Wenguang JING
1
;
Qiwei ZHANG
;
An LIU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Dioscorea; chemistry; Diosgenin; analogs & derivatives; analysis; Drugs, Chinese Herbal; analysis
- From: China Journal of Chinese Materia Medica 2009;34(20):2616-2618
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for the determination of pseudoprodioscin in Dioscorea panthaica and provide scientific basis for quality control of it and its preparation.
METHODThe analysis was carried out on a Merck Purospher STAR RP-18e column eluted with acetonitrile and water as mobile phases in gradient mode. The detection wavelength was 203 nm, and the flow rate was 1.0 mL min(-1).
RESULTThe pseudoprodioscin in D. panthaica extract was well separated. The linear range is between 0.0800-4.00 microg, r =0.9999. The average recovery and RSD are 99.9% and 2.3%, respectively.
CONCLUSIONThis method for quantitation of pseudoprodioscin in D. panthaica is simple, accurate and reliable and can be used for the quality control of D. panthaica and its preparations.
