Determination of scandoside methyl ester in Hedyotis chrysotricha by HPLC.
- Author:
Jing LI
1
;
Guoping ZHOU
;
Caitang LI
;
Ping WEN
;
Ping YING
;
Jinbao YU
;
Huodi WU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; Esters; analysis; Hedyotis; chemistry
- From: China Journal of Chinese Materia Medica 2009;34(20):2619-2621
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for determination of scandoside methyl ester in Hedyotis chrysotricha.
METHODThe determination was carried out on an HC-C18 column elnted with acetonitrile-water (7:93) as mobile phase, and the detection wavelength was set at 238 nm.
RESULTThere is a good linearity in the range 22.08-552 mg L(-1) of scandoside methyl ester and the average recovery is 97.7% (n = 6), RSD 0.72%.
CONCLUSIONThis method is convenient, quick, and is applicable to the quality control of H. chrysotricha.