Screening of anti-tumor parts from Tripterygium hypoglaucum.
- Author:
Mingqing HUANG
1
;
Dongxu JIANG
;
Mingli LUO
;
Shaohua LI
;
Yiping ZHANG
;
Bin JIANG
;
Xiaoping LAI
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; isolation & purification; pharmacology; Animals; Antineoplastic Agents, Phytogenic; isolation & purification; pharmacology; Cell Line, Tumor; Cell Proliferation; drug effects; Drug Screening Assays, Antitumor; Drugs, Chinese Herbal; isolation & purification; pharmacology; Humans; Inhibitory Concentration 50; Mice; Tripterygium; chemistry
- From: China Journal of Chinese Materia Medica 2009;34(20):2633-2636
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen the anti-tumor active parts from Tripterygium hypoglaucum by anti-tumor experimental model in vivo and in vitro.
METHODEthanol extraction was separated and purified by column chromatography of ion polymeric adsorbent and macroporous adsorptive resins. MTT assay and the inhibition effect to S180 solid tumor were used to detect anti-tumor activity of each separation.
RESULTThere are anti-tumor activities in the ethanol extraction, total alkaloids, and macroporous resin absorption in vivo and vitro. Minimum IC50 of Total alkaloids was 29.90 mg L(-1), and S180 solid tumor inhibition ratio of different dose of 25, 50, 100 mg kg(-1) were 38.10%, 50.60%, 60.71% respectively. Minimum IC50 of macroporous resin absorption was 98.56 mg L(-1), and S180 solid tumor inhibition ratio of different dose of 100, 200, 400 mg kg(-1) were 42.96%, 53.57%, 63.79% respectively. Water solubility position had no effect in vivo and vitro.
CONCLUSIONT. hypoglaucum has fine anti-tumor activity in vivo and in vitro, and total alkaloids are the main part of anti-tumor active part.
