Research on technological process of two-pot countercurrent extraction of hydroxysafflor yellow A.
- Author:
Yerui LI
1
;
Yong CHEN
;
Longhu WANG
;
Xueying CHEN
;
Xuesong LIU
;
Changhai SUN
;
Wenjie YAN
Author Information
- Publication Type:Journal Article
- MeSH: Carthamus; chemistry; Chalcone; analogs & derivatives; analysis; isolation & purification; Countercurrent Distribution; methods; Drugs, Chinese Herbal; analysis; isolation & purification; Quinones; analysis; isolation & purification
- From: China Journal of Chinese Materia Medica 2009;34(21):2743-2747
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the optimum technical conditions of extracting Hydroxysafflor yellow A (HSYA) from Carthmus tinctorius by multi-stage countercurrent extraction technology.
METHODThe effects of extraction time of each stage, extraction temperature, ethanol concentration and solid-liquid ratio (g x mL(-1)) on extraction yield of HSYA were studied by orthogonal test design and the comparison of other extraction methods were presented.
RESULTExtraction time and solid-liquid ratio had significant influence on the extraction yield, and the optimum parameters were as follows: Extraction time of each stage was 120 min, solid-liquid ratio was 1 : 10 (g x mL(-1)), ethanol concentration was 30%, and extracted at room temperature. Under the optimum conditions, the extraction yield of HSYA was 1.56% and the purity of the extract was 6.06%. Compared with the traditional extraction method and the ultrasonic extraction method of the pharmacopoeia, the extraction yield was increased by 6.12% and 9.09%, the purity of extract was increased by 42.9% and 27.0%, respectively.
CONCLUSIONThe multi-stage countercurrent extraction technology has many advantages such as simple operation, less solvent consumption, higher extraction yield and purity of extract and it has wide industrial application prospect.
