Simultaneous determination of alpha-pinene, beta-pinene, eucalyptol and alpha-terpineolin in essential oil from Alpinia officinarum Hance by GC.
- Author:
Xiaodi ZHAO
1
;
Xiaohui CHEN
;
Xiaojing TAN
;
Kaishun BI
Author Information
- Publication Type:Journal Article
- MeSH: Alpinia; chemistry; Bridged Bicyclo Compounds; analysis; Chromatography, Gas; methods; Cyclohexanols; analysis; Drugs, Chinese Herbal; analysis; Monoterpenes; analysis; Oils, Volatile; analysis; Terpenes; analysis
- From: China Journal of Chinese Materia Medica 2009;34(21):2751-2753
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for simultaneous determination of alpha-pinene, beta-pinene, eucalyptol and alpha-terpineolin in essential oil from Alpinia officinarum by GC.
METHODThe essential oil was extracted by steam distillation. The determination was carried on with capillary column DB-1 (0.25 mm x 30 m, 0.25 microm). Temperature programs: 50 degrees C (hold 2 min) programmed to 130 degrees C (hold 3 min) at 8 degrees C x min(-1). The detector was FID. Inlet temperature was 230 degrees C. The detector temperature was 250 degrees C. Carrying gas was nitrogen (1.2 mL x min(-1)), split injection was conducted with split ratio of 10:1. Injection volumn was 1 microL.
RESULTThe linear ranges of alpha-pinene, beta-pinene, eucalyptol and alpha-terpineolin were 0.009-0.090 (r = 0.999 8), 0.009-0.091 (r = 0.999 8), 0.060 4-0.604 (r = 0.999 7) and 0.037 4-0.374 g x L(-1) (r = 0.999 5), respectively. The average recoveries (n = 9) of a-pinene, beta-pinene, eucalyptol and alpha-terpineolin were 96.2% (RSD 0.8%) and 96.7% (RSD 1.1%), 98.7% (RSD 1.1%) and 96.7% (RSD 2.2%), respectively.
CONCLUSIONThe developed method is simple, quick and accurate, which is helpful to control the quality of A. officinarum.
