Determination of norisoboldine in Radix Lindera by RP-HPLC.
- Author:
Jianzhong CHEN
1
;
Guixin CHOU
;
Li YANG
;
Changhong WANG
;
Zhengtao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; analysis; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; Lindera; chemistry
- From: China Journal of Chinese Materia Medica 2009;34(21):2774-2776
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a RP-HPLC method for quantitative determination of norisoboldine in Radix Linderae and to provide valuable data for quality control of Radix Linderae.
METHODThe separation was performed on a Phenomenex Gemini C18 column (4.6 mm x 250 mm, 5 microm) at 25 degrees C using a gradient elution of mobile phase A (0.5% formic acid, adjusted pH 2.25 with triethylamine) and mobile phase B (acetonitrile). The detection wavelength was 280 nm.
RESULTThe calibration curve showed a good linearity (r = 0.999 9) within test ranges of 0.015-1.509 microg. The average recovery was 99.58% with RSD 1.4%.
CONCLUSIONThe developed method is simple, accurate and reliable with good repeatability. It is suitable for quality evaluation of Radix Linderae.
