Determination of plasma protein binding rate of bufalin.
- Author:
Ying LIU
1
;
Zhiqiang CHEN
;
Beilei TAO
;
Nianping FENG
;
Jihui ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Proteins; chemistry; Bufanolides; chemistry; Humans; Kinetics; Male; Protein Binding; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2009;34(21):2817-2820
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study plasma protein binding rate of bufalin.
METHODHPLC was employed to determine the concentration of bufalin. Plasma protein binding rate studies were conducted by equilibrium dialysis method. The influence of drug concentration and plasma in different species on plasma protein binding rate were studied.
RESULTThere was no significant difference in the plasma protein binding rates at low, middle and high bufalin concentrations in dilution medium. The protein binding rate of bufalin in human plasma was higher than in the rat plasma.
CONCLUSIONBufalin has higher protein binding extent with both rat plasma and human plasma.
