Simultaneous determination of two anthraquinone-O-glucosides in five processed pieces from Rheum palamatum.
- Author:
Cun ZHANG
1
;
Li LI
;
Yongqing XIAO
;
Na LIN
;
Chunfang LIU
;
Guofang TIAN
;
Dongdong CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Anthraquinones; chemistry; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; chemistry; Glucosides; chemistry; Rheum; chemistry
- From: China Journal of Chinese Materia Medica 2009;34(22):2872-2875
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the contents of two anthraquinone-O-glucosides in five different processed products from Rheum palamatum.
METHODThe content of aleo-emodin-8-O-beta-D-glucoside, rhein-8-O-beta-D-glucoside were determined simultaneously by HPLC on a agilent TC-C18 (2) column at 35 degrees C with the mobile phase of acetonitrile-1% glacial acetic acid (20: 80). The detection wavelength was set at 410 nm and the flow rate was 1.0 mL x min(-1).
RESULTThe calibration curves for two components were linear within the range of 0.017 44-0.348 8 microg (r = 0.999 8, n = 5), 0.084-1.686 microg (r = 0.999 9, n = 5), and the average recoveries were 97.82%, 97.87%, respectively.
CONCLUSIONThe results showed that there were remarkable variation of the two kinds of glucosides contentsamong the five different processed pieces. The contents of the crude pieces, pieces roasted with millet wine and pieces roasted with vinegar were almost the same, while the prepared pieces and charring pieces had evidently reduced contents. The are certain regularity in the variation.
