Intraarticular Injection of Allogenic Mesenchymal Stem Cells has a Protective Role for the Osteoarthritis.
- Author:
Xin YANG
;
Tian-Yue ZHU
;
Li-Cheng WEN
;
Yong-Ping CAO
1
;
Chao LIU
;
Yun-Peng CUI
;
Zhi-Chao MENG
;
Heng LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cartilage, Articular; cytology; Injections, Intra-Articular; Male; Mesenchymal Stem Cell Transplantation; methods; Mesenchymal Stromal Cells; cytology; Osteoarthritis, Knee; prevention & control; therapy; Rats
- From: Chinese Medical Journal 2015;128(18):2516-2523
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDResearchers initially proposed the substitution of apoptotic chondrocytes in the superficial cartilage by injecting mesenchymal stem cells (MSCs) intraarticularly. This effect was termed as bio-resurfacing. Little evidence supporting the treatment of osteoarthritis (OA) by the delivery of a MSC suspension exists. The aim of this study was to investigate the effects of injecting allogenic MSCs intraarticularly in a rat OA model and to evaluate the influence of immobility on the effects of this treatment.
METHODSWe established a rat knee OA model after 4 and 6 weeks and cultured primary bone marrow MSCs. A MSC suspension was injected into the articular space once per week for 3 weeks. A subgroup of knee joints was immobilized for 3 days after each injection, while the remaining joints were nonimmobilized. We used toluidine blue staining, Mankin scores, and TdT-mediated dUTP-biotin nick end labeling staining to evaluate the therapeutic effect of the injections. Comparisons between the therapy side and the control side of the knee joint were made using paired t-test, and comparisons between the immobilized and nonimmobilized subgroups were made using the unpaired t-test. A P value < 0.05 was considered significant.
RESULTSThe three investigative approaches revealed less degeneration on the therapy sides of the knee joints than the control sides in both the 4- and 6-week groups (P < 0.05), regardless of immobilization. No significant differences were observed between the immobilized and nonimmobilized subgroups (P > 0.05).
CONCLUSIONSTherapy involving the intraarticular injection of allogenic MSCs promoted cartilage repair in a rat arthritis model, and 3-day immobility after injection had little effect on this therapy.