Establishment of a quantitative mathematical model of Shen-deficiency syndrome based on whole-genome transcriptional profiles.
- Author:
Zi-yin SHEN
1
;
Xin-min ZHANG
;
Wei LIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Diagnosis, Differential; Drugs, Chinese Herbal; pharmacology; Epimedium; chemistry; Gene Expression Profiling; Kidney Diseases; diagnosis; genetics; prevention & control; Male; Medicine, Chinese Traditional; Models, Theoretical; Rats; Rats, Sprague-Dawley; Syndrome; Yang Deficiency; diagnosis; genetics; prevention & control
- From: Chinese Journal of Integrated Traditional and Western Medicine 2008;28(2):131-134
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a quantitative mathematical model of Shen-deficiency syndrome of TCM by utilizing whole-genome transcriptional profiles.
METHODSThe 4, 10, 18, 24 months old SD rats were used, 24-months aged rats intervened by Epimedium Flavonoids (EF) were adopted in the experiment. Rats' hypothalamus, pituitary, adrenal, lymphocytes, bone, liver, and kidney, and spleen were taken for determining whole-genome mRNA expression with gene chip, and a quantitative nerve network model was established by utilizing the gene expression profile of different aged rats, then the model was used to evaluate the effects of EF on Shen-deficiency syndrome.
RESULTSTotally 199 genes showing age-dependent characteristics were screened out from the 7 kinds of tissue, most of them were neuro-endocrine immune related genes. Evaluation based on the mathematical model showed the age of hypothalamus, pituitary, adrenal, liver, kidney, bone, and spleen in the 24-months rats after EF intervention was 12.64, 10.87, 8.10, 12.70, 11.93, 13.14, and 10.13 months, respectively.
CONCLUSIONA quantitative mathematical model can be established based on the gene expression profile, it is suitable for estimating the efficacy of Shen-tonifying drugs. EF can make the gene expression of elder close to the young state, suggesting that EF has action in improving Shen-deficiency syndrome and delaying senescence.
