Effects of 3 genosides on bio-thermodynamic expression of splenic lymphocyte in mice.
- Author:
Jun-Ling CAO
1
;
Zu-Lun LI
;
Xiao-He XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Survival; drug effects; Cells, Cultured; Energy Metabolism; drug effects; Ginsenosides; pharmacology; Lymphocytes; cytology; drug effects; metabolism; Male; Mice; Mice, Inbred BALB C; Spleen; cytology; Thermodynamics
- From: Chinese Journal of Integrated Traditional and Western Medicine 2008;28(1):54-57
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a new method for screening active ingredients of Chinese herbs by determining different bio-thermodynamic effects of 3 genosides on splenic lymphocyte of mice.
METHODSUsing a thermal bioactivity monitoring system, the maximum heat output (mHO), average metabolic heat (MH) and constant of decrease rate (DR) of lymphocyte were determined based on the growth metabolic power-time curve, and the outcomes were verified by MIT.
RESULTSThe mHO and MH increased and the DR decreased after lymphocytes being exposed to the 3 genosides in different concentrations, arranged upon their potency as genoside Rg3 > genoside Rg2 > genoside Rg1 (merely insignificant effect). MTT showed the same results.
CONCLUSIONHeat activity monitoring system could precisely display the different bio-thermal dynamic effects of 3 genosides on splenic lymphocyte.
