Effect of Bushen Antai Recipe on prostaglandin I2 expression and its nuclear receptor at implantation site in mice with blastocyst implantation dysfunction.
- Author:
Ming-Min ZHANG
1
;
Liang-Liang CHENG
;
Li-Ping DONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Drugs, Chinese Herbal; pharmacology; Embryo Implantation, Delayed; drug effects; Endometrium; drug effects; metabolism; Epoprostenol; biosynthesis; Female; Immunohistochemistry; Male; Mice; PPAR delta; biosynthesis; genetics; Pregnancy; RNA, Messenger; biosynthesis; genetics; Radioimmunoassay; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Integrated Traditional and Western Medicine 2008;28(3):229-233
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of Bushen Antai Recipe (BAR) on expression of prostaglandin I2 (PGI2) and its nuclear receptor peroxisome proliferators-activated receptor delta (PPARdelta) at implantation site in mice with blastocyst implantation dysfunction.
METHODSPregnant mice were divided into three groups randomly, the normal group, the model group and the BAR group. The pregnant uterus of all mice was cut off on the 4th (D4), 5th (D5), 6th (D6) and 8th (D8) day of pregnancy for determining the PGI2 expression with radio immunoassay; and the mRNA and protein expression of PPARdelta with RT-PCR and immunohistochemistry at implantation site.
RESULTSPGI2 expression in the model group was obviously lower than that in the normal group (P < 0.01), and also lower than that in the BAR group (P < 0.01), while the index was insignificantly different between the normal and the BAR group. Compared with the normal group, the expression of PPARdelta in the model group was delayed temporally and spatially (P < 0.05), while that in the BAR group was not significantly different.
CONCLUSIONBAR can improve the implantation in mice with blastocyst implantation dysfunction through promoting the PGI2 expression and its nuclear receptor PPARdelta at implantation site.
