Regulatory effect of ginsenoside on glucocorticoid receptor in mice with ischemic liver damage.
- Author:
Ying-Lu FENG
1
;
Bin-Bin CHENG
;
Chang-Quan LING
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Gene Expression Regulation; drug effects; Ginsenosides; pharmacology; Ischemia; physiopathology; Liver; blood supply; drug effects; metabolism; Male; Mice; RNA, Messenger; biosynthesis; genetics; Random Allocation; Receptors, Glucocorticoid; genetics; Reverse Transcriptase Polymerase Chain Reaction; Time Factors
- From: Chinese Journal of Integrated Traditional and Western Medicine 2008;28(3):252-254
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study whether ginsenoside (GS) can regulate the glucocorticoid receptor (GR) in mice with ischemic liver damage, and to preliminarily observe its dose-effect relationship for providing an experimental bases in seeking a new way to relieve the damage from view of GR.
METHODSAdult male SD mouse was used to establish liver ischemia model, and different doses (100, 50, and 25 mg/kg) of GS was given via gastric infusion before modeling. The maximal GR binding capacity (Bmax) of liver and the level of GR mRNA expression in liver were dynamically determined at various time points (2 h, 6 h, 12 h and 24 h) after modeling.
RESULTSCompared with the normal control group, GR Bmax and GR mRNA expression in model rats were lower obviously (P < 0.01). As compared with the control group, GR Bmax and GR mRNA expression in model rats treated with 50 mg/kg GS significantly raised at 2 h, 6 h, 12 h (P < 0.01), while the changes in modeling rats treated with other two doses of GS were of no statistical significance.
CONCLUSIONGS in dose of 50 mg/kg can elevate the GR Bmax of liver and the level of GR mRNA expression in liver of rats with ischemic damage.
