Diagnostic value of SYT-SSX fusion gene detection by fluorescence in-situ hybridization for synovial sarcoma.
- Author:
Yan SUN
1
;
Bao-Cun SUN
;
Yan-Xue LIU
;
Shi-Wu ZHANG
;
Xiu-Lan ZHAO
;
Jian WANG
;
Xi-Shan HAO
Author Information
- Publication Type:Journal Article
- MeSH: Biomarkers, Tumor; analysis; genetics; Humans; In Situ Hybridization, Fluorescence; methods; Nucleic Acid Hybridization; methods; Oncogene Proteins, Fusion; genetics; isolation & purification; Pathology, Molecular; methods; Reverse Transcriptase Polymerase Chain Reaction; Sarcoma, Synovial; diagnosis; genetics; Soft Tissue Neoplasms; diagnosis; genetics
- From: Chinese Journal of Pathology 2008;37(10):660-664
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method of SYT-SSX fusion gene detection by FISH and to explore its diagnostic value for synovial sarcoma.
METHODSThe presence of SYT-SSX fusion gene was determined by FISH using a tissue microarray containing 62 known synovial sarcomas, 60 non-synovial sarcomas and 133 equivocal synovial sarcomas. FISH results were compared with those of RT-PCR published previously.
RESULTSOverall, 96.9% (247/255) of the cases were successfully analyzed by FISH. The sensitivity of FISH for known synovial sarcomas was 96.7% (58/60), and the specificity for the non-synovial sarcoma was 100% (59/59). Moreover, SYT-SSX gene fusion was detected in 78.1% (100/128) of the equivocal synovial sarcomas. The concordance rate between FISH and RT-PCR was 83.6% (102/122) in those equivocal synovial sarcomas, and overall 79.7% (106/133) of these cases were confirmed as synovial sarcomas either by RT-PCR or by FISH.
CONCLUSIONSThe sensitivity and specificity of FISH detection of SYT-SSX fusion gene are high. FISH and RT-PCR are complementary to each other in the confirmation of synovial sarcomas, particularly those questionable cases.
