Diversity and in vitro antitumnor activity of endophytic fungi from mangrove plants Xylocarpus.
- Author:
Ning LI
1
;
Fei-Ying RUAN
;
Zheng-Shun WEN
;
Jian-Hua LI
;
Ri-Dao CHEN
;
Xiao LIU
;
Dan XIE
;
Min-Yi LI
;
Chun-Mei WANG
;
Jun WU
;
Jun-Gui DAI
Author Information
1. College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100102, China. ningjianger@163.com
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
isolation & purification;
pharmacology;
Biodiversity;
Cell Line, Tumor;
Endophytes;
chemistry;
classification;
genetics;
isolation & purification;
Fungi;
chemistry;
classification;
genetics;
isolation & purification;
HCT116 Cells;
Humans;
Meliaceae;
microbiology;
Phylogeny
- From:
China Journal of Chinese Materia Medica
2013;38(14):2282-2286
- CountryChina
- Language:Chinese
-
Abstract:
A total of 24 biologically pure entophytic fungal strains were isolated from stems, leaves, and seed coats of Xylocarpus plants by repeated purification, and identified with Internal Transcribed Spacer (ITS) rDNA molecular method, which belonging to 14 genera, 11 families, 9 orders and 3 classes. There were differences in genus and species levels among three plant materials from different habitats and species, and it was found that the strains of Phomopsis and Colletotrichum existed in all three plant materials. In vitro assay of antitumor activity by MTT method revealed that the EtOAc extracts of 15 strains exhibited potent antitumor activity. These results suggest that it is of value for further investigation on the above fungal strains.
