Simultaneous determination of six ingredients in Huoxiang Zhengqi oral liquid by UPLC.
- Author:
Jia-Wen YU
1
;
Kai-Ying DENG
;
Tao PENG
;
Bi-Yue ZHU
;
Hong-Ya LIU
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Oral; Biphenyl Compounds; chemistry; Chromatography, High Pressure Liquid; methods; Disaccharides; chemistry; Drugs, Chinese Herbal; chemistry; Flavanones; chemistry; Glucosides; chemistry; Hesperidin; chemistry; Lignans; chemistry; Pharmaceutical Solutions; chemistry
- From: China Journal of Chinese Materia Medica 2013;38(14):2314-2317
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a UPLC method for the simultaneous determination of liquiritin, narirutin, hesperidin, ammonium glycyrrhetate, honokiol and magnolol in Huoxiang Zhengqi oral liquid.
METHODA Zorbax Eclipse C18 column was used with the mobile phase of acetonitrile and 0. 05% phosphate acid by gradient elution at the detection wavelength of 220 nm. The flow rate was 0.42 mL x min(-1) and the column temperature was 30 degrees C.
RESULTThe calibration curves were linear in the ranges of 0.001 7-0.034, 0.003 4-0.068, 0.006 4-0.128, 0.012 8-0.256, 0.003 2-0.064, 0.006 4-0.128 microg, respectively. The average recoveries were 103.3%, 98.39%, 98.29%, 102.1%, 98.45%, 102.2% with RSDs of 2.1%,1.0%, 0.50%, 2.3%, 0.9%, 2.0%, respectively.
CONCLUSIONThe UPLC method was simple, rapid and accurate, it could be used for quality control of Huoxiang Zhengqi oral liquid.
