Spectrum-effect relationship between anti-HIV 1 activities and ultra-performance liquid chromatography fingerprints of Rheum species.
- Author:
Pei MA
1
;
Xin-Yao ZHANG
;
Li-Jia XU
;
Zhe WANG
;
Pei-Gen XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Anti-HIV Agents; chemistry; pharmacology; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; chemistry; pharmacology; HIV Reverse Transcriptase; metabolism; HIV-1; drug effects; enzymology; Inhibitory Concentration 50; Rheum; chemistry; Ribonuclease H, Human Immunodeficiency Virus; metabolism; Structure-Activity Relationship
- From: China Journal of Chinese Materia Medica 2013;38(15):2434-2437
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the relationship between ultra-performance liquid chromatography (UPLC) fingerprints of Rheum species and their anti-HIV 1 activities.
METHODTwenty two samples of 16 species belonging to genus Rheum from various sources were collected and analyzed in this study. Firstly they were assayed for the HIV-1 reverse transcriptase (RT) associated ribonuclease H (RNase H) activity. Secondly the fingerprints were established by an optimized UPLC method. Sample was analyzed by UPLC-TOF-MS/MS to identify major peaks. The possible relationship between UPLC fingerprints and anti-HIV 1 activities of Rheum species were deduced by mathematical statistics method.
RESULTSamples of R. austral, R. austral, R. hotaoense exhibited good anti-HIV 1 activities with IC50 < or = 0.2 mg x L(-1). The correlation of anti-HIV 1 activities and fingerprints showed that three compounds were the main bioactive components, and their retention times were 4.74, 7.99, 21.18 min, respectively.
CONCLUSIONCompounds in Rheum species with possible anti-HIV 1 activities were deduced by spectrum-effect relationship study. This study supported for study of medicinal plants in Rheum.
