Authenticate of Atractylodes macrocephala seed by amplification refractory mutation system.
- Author:
Liang CAO
1
;
Chao JIANG
;
Hua-Sheng PENG
;
Min CHEN
;
Yuan YUAN
Author Information
- Publication Type:Journal Article
- MeSH: Atractylodes; genetics; DNA Primers; genetics; Mutation; Polymerase Chain Reaction; methods; Quality Control; Seeds; genetics
- From: China Journal of Chinese Materia Medica 2013;38(16):2567-2570
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo design specific primers and authenticate Atractylodes macrocephala from Atractylodes lancea and A. chinensis.
METHODSNPs in the psbA-trnH sequences of Atractylodes were found by ClustulW program and Bioedit software. Primers for authentic A. macrocephala is designed according to the SNP site, and ITS sequence universal primers plus to the authentic primer to construct a multi-PCR reaction system, and then optimized the PCR reaction system.
RESULT172 bp band special for A. macrocephala were found using multi-PCR reaction.
CONCLUSIONThe multi-PCR reaction system could be applied to identify A. macrocephala seed.
