Physicochemical properties and skin penetration in vitro of total alkaloids of Sophora flavescens nanoemulsion.
- Author:
Ai-Ling FENG
1
;
Ying-Zi WANG
;
Sheng-Hai ZHANG
;
Xiu-Yu SUN
;
Fei-Peng DUAN
;
Cai-Xia LI
Author Information
1. School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. fengailing6167@sina.com
- Publication Type:Journal Article
- MeSH:
Alkaloids;
chemistry;
metabolism;
Animals;
Chemical Phenomena;
Drug Carriers;
chemistry;
Emulsions;
Male;
Nanostructures;
chemistry;
Rats;
Rats, Sprague-Dawley;
Skin Absorption;
Sophora;
chemistry
- From:
China Journal of Chinese Materia Medica
2013;38(16):2628-2632
- CountryChina
- Language:Chinese
-
Abstract:
The research aimed at investigating the physicochemical properties, stability and skin penetration in vitro of total alkaloids of Sophora flavescens nanoemulsion. Prepare total alkaloids of S. flavescens nanoemulsion and detect the determination of matrine and oxymatrine in the nanoemulsion using HPLC method. Transmission electron microscopy and laser particle size analyzer were utilized to detect the shape and size of the nanoemulsion respectively. And also the stability of nanoemulsion was studied under the conditions of low temperature (4 degrees C), normal temperature (25 degrees C) and high temperature (60 degrees C). Franz diffusion cell was used to research the transdermal absorption of nanoemulsion in vitro. The results found that the nanoemulsion we prepared presented appearance of rounded, uniform; its average diameter was (15.55 +/- 2.24) nm, and particle size distribution value was 0. 161; the appearance, diameter and percentage determination of total alkaloids of S. flavescens had no variations after 15 d under 4, 25, 60 degrees C respectively. The steady-state permeation rate was 4.564 1 microg x cm(-2) x h(-1), 24 h cumulative amount of penetration was 110.7 microg x cm(-2), which was 1.86 fold of 24 h cumulative amount of aqueous solution (59.41 microg x cm(-2)). All the results demonstrated total alkaloids of S. flavescens nanoemulsion had good permeability, and could provide a new preparation for its clinical application.
