Study on quality control of Houttuynia Cordata, a traditional Chinese medicine by fingerprint combined with quantitative analysis of multi-components by single marker.
- Author:
Bing HE
1
;
Yan LIU
;
Ji TIAN
;
Chun-Hong LI
;
Shi-Yan YANG
Author Information
1. Research Center for Drug, Luzhou Medical College, Luzhou 646000, China. lyhb2008@126.com
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
methods;
standards;
Drugs, Chinese Herbal;
analysis;
chemistry;
Quality Control;
Reproducibility of Results
- From:
China Journal of Chinese Materia Medica
2013;38(16):2682-2689
- CountryChina
- Language:Chinese
-
Abstract:
To establish a new method of quality evaluation of Traditional Chinese medicine by fingerprint and quantitative analysis of multi-components by single marker method (QAMS). The quality evaluation method was established and validated with Houttuyniae Herba. Chlorogcnic acid was selected as markers of ingredients to establish HPLC fingerprint and internal reference standard to determine the contents of other 6 components (new chlorogcnic acid, cryptochlorogenic acid, rutin, hyperin, isoquercitrin, quercitrin) according to the relative correction factor. At the same time, the seven components were determined by external standard method. The accuracy and feasibility of QAMS was evaluated by comparison of the results between external standard method and QAMS. All tested samples contained the 12 common peaks , 7 of which was verified ,and there was no significant differences between the quantitative results of 7 ingredients of multi-components by single marker method and external standard method in 20 batches. The method of fingerprint combined with QAMS has been verified in Houttuyniae Herba and it is to be a new quality evaluation pattern for Traditional Chinese medicine.
