Effect of oxymatrine on JAK2/STAT3 signaling in renal tissues of rats with septic shock.
- Author:
Xiu-Yu WANG
1
;
Ming-Hao ZHANG
;
Mei-Ling YANG
;
Yi-Deng JIANG
;
Gui-Zhong LI
;
Xiao-Ling YANG
;
Hua XU
;
Jun CAO
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; pharmacology; Animals; Gene Expression Regulation; drug effects; Interleukin-1beta; genetics; metabolism; Janus Kinase 2; metabolism; Kidney; drug effects; metabolism; pathology; Male; Quinolizines; pharmacology; Rats; Rats, Sprague-Dawley; STAT3 Transcription Factor; metabolism; Shock, Septic; blood; metabolism; pathology; Signal Transduction; drug effects; Tumor Necrosis Factor-alpha; genetics; metabolism
- From: China Journal of Chinese Materia Medica 2013;38(16):2696-2700
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of oxymatrine (OMT) on JAK2/STAT3 signaling in renal tissues of rats with septic shock.
METHODThe cecal ligation and puncture (CLP) was adopted to establish the rat septic shock model. Fifty-six male SD rats were randomly divided into 7 groups: the sham operation group, the model (CLP) group, CLP + OMT high, middle, low-dose (52, 26, 13 mg x kg(-1), vena caudalis bolus) groups and the positive control (CLP + dexamethasone, 10 mg x kg(-1)) group. The pathological changes in renal tissues were examined with lightmicroscope. BUN content was determined by urine enzymatic method. Expressions of tumournecrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) mRNA in renal tissues were determined by RT-PCR. Expression of JAK2 and STAT3 in renal tissues determined by Western blot. Changes in tumournecrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) contents in renal tissue were determined by radioimmunoassay.
RESULTOMT of different doses could inhibit the JAK2 and STAT3 activation in renal tissues (P<0.05), and decrease the protein expression of JAK2, STAT3, TNF-alpha and IL-1beta mRNA (P<0.05). Besides, it could reduce TNF-alpha and IL-1beta contents in renal tissue homogenate (P<0.05), serum BUN content (P<0.05), and improve such lesions as tissue hyperemia, edema and inflammatory cell infiltration, with identical results in medium and high-dose OMT groups, and the positive control group.
CONCLUSIONOMT can inhibit JAK2/STAT3 signaling activity to reduce the expression of proin-flammatory factors (TNF-alpha, IL-1beta) and treat the renal injury in rats with septic shock.
