Gene diagnosis of hemophilia B by multiple STR analysis.
- Author:
Xiangfan LIU
1
;
Xuefeng WANG
;
Qishi FAN
;
Haiyan CHU
;
Yi FANG
;
Hongli WANG
Author Information
- Publication Type:Journal Article
- MeSH: Female; Genetic Carrier Screening; methods; Genetic Linkage; Genetic Predisposition to Disease; Hemophilia B; diagnosis; genetics; Humans; Male; Microsatellite Repeats; genetics; Pedigree; Polymorphism, Genetic
- From: Chinese Journal of Hematology 2002;23(3):147-150
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a simple, rapid genetic diagnostic system for haemophilia B.
METHODSThe polymorphisms of eight STR loci in 87 normal persons and 8 haemophilia B families were assayed by PCR and genescan, and the linkage relations were analysed.
RESULTSSix of the eight STR loci can provide genetic information for haemophilia B, and the heterozygosity is 0.50 approximately 0.83, PIC 0.39 approximately 0.80, and DP 0.66 approximately 0.94.
CONCLUSIONCombination of multiple STR loci analysis could be effective method for genetic diagnosis of haemophilia B.
