Construction and expression of a vector containing protein transduction domain and bcr/abl fusion gene.
- Author:
Yingmin LIANG
1
;
Qiang SUN
;
Shanshan JIANG
;
Jizhu WANG
;
Rongli WU
;
Ping CHEN
;
Li LIU
;
Hua HAN
Author Information
- Publication Type:Journal Article
- MeSH: Electrophoresis, Polyacrylamide Gel; Escherichia coli; genetics; Fusion Proteins, bcr-abl; genetics; metabolism; Gene Expression; Gene Products, tat; genetics; metabolism; Genetic Vectors; genetics; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; genetics; Recombinant Fusion Proteins; genetics; metabolism
- From: Chinese Journal of Hematology 2002;23(1):5-8
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct a vector containing protein transduction domain (PTD) and bcr/abl fusion gene of chronic myelogenous leukemia and express PTD-bcr/abl fusion protein in E. Coli.
METHODSDNA fragment encoding PTD was synthesized and fused to PCR-amplified bcr/abl gene fragment, then inserted into plasmid pET-16b to get the expression vector pEPb containing PTD-bcr/abl fusion gene, which was transfected and expressed in E. Coli LB21. PTD-bcr/abl fusion protein was purified by affinity chromatography.
RESULTS523 bp bcr/abl fusion gene was effectively amplified. The PTD-bcr/abl gene sequencing showed the same sequence as scheduled. The fusion peptide was successfully expressed in E. Coli and purified.
CONCLUSIONThe results may provide a new PTD-bcr/abl fusion peptide for the immunotherapy of CML.
