Study on expression of serum concentration of antibody IgA1 in normal people and patients with hepatocellular epithelioma by j1-sepharose 4b-lectin affinity column chromatography
- Author:
Loi Van Nguyen
- Publication Type:Journal Article
- Keywords:
IgA
- MeSH:
Immunoglobulin A;
Chromatography
- From:Pharmaceutical Journal
2000;288(4):16-18
- CountryViet Nam
- Language:Vietnamese
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Abstract:
We have used jacalin, a lectin purified from Vietnamses Jackfruit to design J1- sepharose 4 B affinity column for studying IgA1 concentration from healthy and Hepato Cellular Carcinoma sera. The results obtained showed that IgA1 concentration from human Hepato Cellular Carcinoma serum (7.85+/- 0.80 mg/ml) is 2.76 times higher than that of healthy human (2.84+/-0.28 mg/ml). The use of modified ELISA technique for determination IgA1 concentration from sera indicated the same results in comparison with Affinity chromatography technique.
