Noninvasive prenatal diagnosis of Hb Bart's hydrops fetus using cell-free fetal DNA in maternal plasma.
- Author:
Xing-Jiang LONG
1
;
Gui-Fang LONG
;
Wei-Xiong LIN
Author Information
- Publication Type:Journal Article
- MeSH: Bartter Syndrome; diagnosis; DNA; blood; Female; Humans; Hydrops Fetalis; diagnosis; Molecular Sequence Data; Pregnancy; Pregnancy Trimester, First; Pregnancy Trimester, Second; Pregnancy Trimester, Third; Prenatal Diagnosis; methods
- From: Chinese Journal of Hematology 2009;30(3):175-178
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore a new noninvasive method for Hb Bart' s hydrops fetus by using PCR amplification efficiency discrimination between cell-free fetal DNA (cffDNA) and cell-free maternal DNA in maternal plasma.
METHODSCffDNA samples from pregnant women bearing possible Hb Bart's hydrops fetus were collected. Fluorescent PCR and capillary electrophoresis (CE) were performed. Hb Bart's hydrops fetus was conclusively identified by different peak area ratio of products.
RESULTSThe peak area ratio of 30 cffDNA samples from Hb Bart' s hydrops fetus was much less than 1. However, the ratio of cffDNA sample from hydrops fetus due to other reasons was approximately equal to 1.
CONCLUSIONBy using cffDNA fluorescent PCR and CE, a prenatal screening method for Hb Bart' s hydrops fetus was developed.
