Effects of Annexin II gene silencing by siRNA on proliferation and invasive potential of Jurkat lymphoma cells.
- Author:
Hong-yu BAO
1
;
Miao JIANG
;
Zhen-ni MA
;
Fei SHENG
;
Ming-qing ZHU
;
Lin CHEN
;
Li-qian XIE
;
Ning-zheng DONG
;
Chang-geng RUAN
Author Information
- Publication Type:Journal Article
- MeSH: Annexin A2; genetics; metabolism; Cell Proliferation; Chemotaxis; genetics; Gene Silencing; Humans; Jurkat Cells; RNA, Small Interfering; genetics; Transfection
- From: Chinese Journal of Hematology 2009;30(5):303-306
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of Annexin II (AnxA2) gene silencing by siRNA on proliferation and invasive potential of lymphoma cell line Jurkat cells.
METHODSA synthesized siRNA duplex targeting to AnxA2 was transfected into Jurkat cells. Transfection efficiency was analyzed by real-time PCR and flow cytometry. MTT assay for cell proliferation and transwell plates for invasive potential were performed.
RESULTSCompared with the negative controls, the cell proliferation inhibitory rate of the AnxA2 siRNA transfected Jurkat cells was significantly increased at 24 h, 48 h and 72 h [(17.4 +/- 2.3)%, (22.4 +/- 3.8)%, (37.6 +/- 1.5)% vs (-1.3 +/- 5.1)%, (-5.5 +/- 4.4)%, (-10.8 +/- 5.5)%, respectively, P<0.05]. The cell invasive potential of the transfected Jurkat cells was inhibited remarkably at 48 h (11.3 +/- 4.2 vs 54.3 +/- 8.7, P<0.01).
CONCLUSIONAnxA2 gene silenced by siRNA can inhibit the proliferation and the invasive potential of Jurkat cells remarkably.
