Prognostic factors for chronic lymphocytic leukemia with typical and atypical immunophenotype.
- Author:
Xin CAO
1
;
Wei XU
;
Yu-Jie WU
;
Chun QIAO
;
Qiong LIU
;
Lei FAN
;
Kou-Rong MIAO
;
Jian-Yong LI
Author Information
- Publication Type:Journal Article
- MeSH: ADP-ribosyl Cyclase 1; metabolism; Adult; Aged; Aged, 80 and over; Female; Humans; Immunoglobulin Heavy Chains; genetics; Immunoglobulin Variable Region; genetics; Immunophenotyping; Leukemia, Lymphocytic, Chronic, B-Cell; genetics; immunology; metabolism; Male; Middle Aged; Mutation; Neoplasm Staging; Prognosis; ZAP-70 Protein-Tyrosine Kinase; metabolism
- From: Chinese Journal of Hematology 2009;30(7):450-453
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the prognostic factors for chronic lymphocytic leukemia (CLL) with typical and atypical immunophenotype. The parameters analyzed included sex, age, Binet stages, absolute lymphocyte count (ALC), immunoglobulin heavy-chain variable region (IgVH) gene mutation status, ZAP-70 protein, CD38 expression and cytogenetic aberrations.
METHODSAccording to the clinical guideline and scoring system for CLL in Britain, among 77 patients, 61 patients with score 5 called typical immunophenotype CLL, 16 with score 4 or 3 were atypical immunophenotype CLL. Multiparameter flow cytometry was employed for immunophenotypic analysis in 77 CLL patients for CD5, CD19, CD23, FMC7, sIg, CD20, CD79b expression and ZAP-70 protein and CD38. IgVH mutation status was detected by multiplex RT-PCR and sequencing of the purified PCR amplification products. Fluorescence in situ hybridization (FISH) and a panel of probes were used to detect cytogenetic aberrations.
RESULTSThere was no significant difference between the two groups in sex, age, ZAP-70 and IgVH mutation status (P=0.398, P=0.189, P=0.268 and P=0.131, respectively). The incidence of ALC> or =50 x 10(9)/L, Binet B + C, CD38> or =30% in atypical CLL patients (43.8%, 87.5% and 43.8%, respectively) were higher than that in typical group (16.4%, 36.1% and 16.4%, respectively) (P=0.026, P<0.01 and P=0.026, respectively). The proportion of typical patients (26.8%) with a 13q14 deletion as sole abnormality was higher than that of atypical patients (7.6%), and that with deletion of 11q22 or 17p13 was lower than that of atypical patients (12.2% vs 46.2%) (P=0.022).
CONCLUSIONThere were obvious differences between the typical immunophenotype CLL and atypical CLL in ALC, Binet stages, CD38 expression level and cytogenetic aberrations.