CpG-oligodeoxynucleotide stimulation improves the success for karyotypic analysis of chronic lymphocytic leukemia cells.
- Author:
Qiong LIU
1
;
Wei XU
;
Hai-rong QIU
;
Rong WANG
;
Hui YU
;
Lei FAN
;
Kou-rong MIAO
;
Jian-yong LI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Chromosome Aberrations; Chromosome Banding; Female; Humans; Karyotyping; Leukemia, Lymphocytic, Chronic, B-Cell; genetics; pathology; Male; Middle Aged; Oligonucleotides; pharmacology; Tumor Cells, Cultured
- From: Chinese Journal of Hematology 2009;30(9):601-604
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of CpG-oligodeoxynucleotides (ODN) in chromosome study of chronic lymphocytic leukemia (CLL).
METHODSBlood or bone marrow cells of 70 CLL patients were cultured for 72 h with PHA, CpG-ODN and CpG-ODN combined with IL-2, respectively. Routine karyotype analysis with R banding technique and interphase fluorescence in situ hybridization (FISH) were performed.
RESULTSThe metaphase number>or=20 was considered as successful stimulation, which in PHA, CpG-ODN and CpG-ODN combined IL-2 groups were 90.0%, 68.6% and 68.6%, respectively, and the detection rates of chromosome aberrations were 3.2%, 43.6% and 43.6%, respectively. The aberrations rates detected by interphase FISH with a panel of probes was 64.3%.
CONCLUSIONCpG-ODN DSP30 can effectively raise the detection rate of chromosome aberrations in CLL patients.
