The experimental study on the effect of CDMP-1 on proliferation of residual ear chondrocytes of microtia cultured in vitro.
- Author:
Yu-Zhu QUAN
1
;
Hong-Xing ZHUANG
;
Tun LIU
;
Feng XUE
;
Bo PAN
;
Yan-Yong ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Cell Differentiation; Cell Proliferation; drug effects; Cells, Cultured; Child; Child, Preschool; Chondrocytes; cytology; drug effects; Ear Deformities, Acquired; Female; Growth Differentiation Factor 5; pharmacology; Humans; Male
- From: Chinese Journal of Plastic Surgery 2007;23(3):241-243
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the alternation of biological characteristics of residual ear chondrocytes when proliferating in vitro, and study the effects of CDMP-1 on proliferation and differentiation of residual ear chondrocytes.
METHODSResidual ear chondrocytes were cultured in vitro. Then we observed the effect of CDMP-1 on differentiation by immunohistochemistry of collagen type II and dyeing with toluidine blue, investigated proliferation effects of CDMP-1 by method of MIT, and analysis cell cycle changes by flow cytometry.
RESULTSThe quantity of GAG gradually decreased along with the increase of the doubling time of chondrocytes in vitro. The quantity of chondrocytes with positive expression of collagen type II were significantly more in the experimental group used with CDMP-1 than the contrast. And the most efficient concentration of CDMP-1 was 100 ng/ml.
CONCLUSIONSCDMP-1 had a good effect on proliferating and could maintain morphology of the residual ear chondrocytes of microtia cultured in vitro.
