Experimental study of construction of tissue engineered bone ectopically by human bone marrow mesenchymal stem cells.
- Author:
Dong LI
1
;
Xiang-dong LIU
;
Gang CHAI
;
Chao-feng SHU
;
Wei LIU
;
Lei CUI
;
Yi-lin CAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; cytology; Bone and Bones; Cell Adhesion; Cell Differentiation; Humans; Mesenchymal Stromal Cells; cytology; Mice; Mice, Nude; Tissue Engineering; methods
- From: Chinese Journal of Plastic Surgery 2007;23(5):409-411
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the possibility and mechanism of construction of tissue engineered bone with human bone marrow mesenchymal stem cells (hBMSCs) as seeding cells and partially demineralized bone matrix (pDBM) as scaffold.
METHODShBMSCs are cultured and mutiplified. The 4th grade hBMSCs are seeded on the pDBM, the growth and adhesion of hBMSCs on pDBM are observed under scanning electro microscope. The adhesion efficiency is assessed. The complexes are implanted in the nude mice subcutaneously, the pDBM without cells as control. The grafts are taken out on the 8th and 12th week.
RESULTSThere is new bone formation on the 8th and 12th week in complex group. There is a layer of osteoblast like cells adhered on the surface of most of the new bone, which suggest the possibility of intramembranous ossification. There is no bone formation in control group.
CONCLUSIONSTissue engineered bone can be constructed with hBMScs and pDBM in vivo, and the mechanism of which could be intramembranous ossification.
