The experimental study of creating a new rat scarring model by inserting absorbable gelatin sponge into rats' excisional wounds.
- Author:
Xiao-Li WU
1
;
Wei LIU
;
Zhen GAO
;
Ze-Hao CAI
;
Dan DENG
;
Li-Juan ZONG
;
De-Min YIN
;
Yi-Lin CAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cicatrix; pathology; Dermatologic Surgical Procedures; Disease Models, Animal; Gelatin Sponge, Absorbable; Male; Rats; Rats, Sprague-Dawley; Skin; pathology; Suture Techniques; Wound Healing
- From: Chinese Journal of Plastic Surgery 2008;24(1):42-45
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the possibility of creating a rat new scar model by inserting gelatin sponge into rat excisional wounds.
METHODSTwo full-thickness wounds were created in each of total 49 SD rats. In the Experimental group (n = 19), a regular incisional wound (1 cm) was created on the left side, and an excisional wound of 1.0 cm x 0.2 cm was created on the right side with a gelatin sponge inserted. In control 1 group (n = 15), an excisional wound with sponge insertion was created on both sides of rats. In control 2 group (n = 15), two excisional wounds were created on both sides, and only one side wounds were inserted with a sponge. Animals were sacrificed at various time points for different examinations.
RESULTSThe wound/scar width increased 4 - 11 times in inserted wounds than in regular incisional wounds (P < 0.01), with an obvious delay of epithelialization. No difference in wound/scar width was found in both sides of wounds of control 1 group at various locations. In contrast to the linear scar of sponge-inserted wounds, contracted and irregular scar was found in non-inserted wounds of control 2 group.
CONCLUSIONSGelatin sponge insertion can create a thick linear scar in rat wounds, and thus provides a new model for scar research.