Suppressive effect of rhIL-24 protein on the human A375 cell melanoma in nude mouse.
- Author:
Xiao-Yu ZHAO
1
;
Su YAN
;
Mei HAN
;
Wei-Hua SHENG
;
Ji-Cheng YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Cell Line, Tumor; Female; Humans; Interleukins; pharmacology; Melanoma, Experimental; drug therapy; Mice; Mice, Inbred BALB C; Mice, Nude; Recombinant Proteins; pharmacology
- From: Chinese Journal of Plastic Surgery 2008;24(1):54-57
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the suppressive effect of purified and renaturalized rhIL-24 protein on the human A375 cell melanoma in nude mouse.
METHODSHuman A375 cells were injected into the nude mouse. After the volume of tumor attained, rhIL-24 was injected into the tumor. 2 weeks later, the tumors were resected for measurement of volume and weight, following with pathological and immunohistochemistry examination.
RESULTSThe volume and weight of tumors were decreased markedly after treatment of rhIL-24, when compared with those in controls. The expression of Bax gene upregulated, while Bcl-2, CD34 and VEGF gene downregulated. It indicated tumor growth inhibition and inducing of apoptosis of tumor cells.
CONCLUSIONSrhIL-24 has a suppressive effect on the A375 cell melanoma in nude mouse. It can also induce the A375 cell apoptosis without side effect on nude mouse.
