Study on the therapeutic mechanism of the active principle of the Chinese drug Paeoniae Radix 801 through affinity biosensors IAsys plus quartz crystal microbalance.
- Author:
Jia-dong HUANG
1
;
Zhao SONG
;
Jing LI
;
Bao-yan WU
;
Hui-jun YIN
;
Ke-ji CHEN
;
Qiang CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Biosensing Techniques; standards; Blood Circulation; drug effects; Drugs, Chinese Herbal; metabolism; therapeutic use; Endothelin-1; metabolism; Humans; Quartz
- From:Chinese journal of integrative medicine 2005;11(1):37-40
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the targeted point and mechanism of the function of the blood-activating and stasis-removing Chinese drugs, Paeoniae Radix 801(PR801) in its cardiovascular protective effects and its specific binding with endothelin 1 (ET-1) as well as the dynamics of the two's interactive function by means of using affinity biosensors: IAsys Plus and quartz crystal microbalance (IAQCM).
METHODSET-1 was immobilized on the surfaces of IAQCM by using the new surface modification methods. The PR801 in the solution was detected by modified substrates and the specific binding between PR801 and ET-1 was studied.
RESULTSThe curves went up or down after adding PR801. There is specific binding between PR801 and ET-1. The bound mass were 0.458 ng/mm(2) and 133.54 ng/cm(2), respectively. There exists relatively good stability with these two methods.
CONCLUSIONThe affinity biosensors: IAQCM can be used to study the interaction mechanism between PR801 and ET-1, providing a new way to study the interaction mechanism of TCM. PR801 can bind ET-1 specifically in the experiments. Therefore, ET-1 is another target that PR801 can bind specifically besides thromboxane A(2).
