Effects of total flavone of Abelmoschl Manihot L. Medic on the function of platelets and its mechanism.
- Author:
Yan GUO
1
;
Li FAN
;
Liu-yi DONG
;
Zhi-wu CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Platelets; drug effects; Calcium; blood; Calcium Channel Blockers; administration & dosage; pharmacology; Calcium Chloride; pharmacology; Carotid Artery Thrombosis; blood; etiology; Collagen; pharmacology; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; administration & dosage; pharmacology; Flavones; administration & dosage; pharmacology; Glycosides; administration & dosage; pharmacology; Intracellular Membranes; metabolism; Osmolar Concentration; Platelet Aggregation; drug effects; Platelet Aggregation Inhibitors; administration & dosage; pharmacology; Platelet Function Tests; Rabbits; blood; Rats; Rats, Wistar
- From: Chinese journal of integrative medicine 2005;11(1):57-59
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the effects of total flavone of Abelmoschl Manihot L. Medic (TFA) on the function of platelets and to explore its mechanism.
METHODSRat models of artery-veins bypassing thrombus formation were used. The platelets of rabbits were collected. Platelet aggregation was induced by collagen and intracellular calcium ion concentration ([Ca(2+)]i) was assayed by Fura-2 method.
RESULTSTFA (25, 50, 100 mg/kg) significantly and dose-dependently reduced the weight of thrombus. TFA (0.025, 0.05, 0.1 mg/ml) possessed dose-dependant inhibitory effects on rabbits' platelet aggregation induced by collagen. TFA significantly reduced the resting and CaCl(2)-induced increase of free intracellular calcium concentration ([Ca(2+)]i) in rabbit platelet in vitro.
CONCLUSIONTFA has an antiplatelet effect via the inhibition on the influx of Ca(2+).
