Attenuation of brain inflammatory response after focal cerebral ischemia/reperfusion with Xuesaitong injection in rats.
- Author:
Wei HE
1
;
Xiao-jun XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain Edema; drug therapy; immunology; Brain Ischemia; drug therapy; immunology; Encephalitis; drug therapy; immunology; Intercellular Adhesion Molecule-1; metabolism; Male; Neuroprotective Agents; pharmacology; Neutrophils; immunology; Peroxidase; metabolism; Rats; Rats, Wistar; Reperfusion Injury; drug therapy; immunology; Saponins; pharmacology
- From: Chinese journal of integrative medicine 2006;12(3):203-206
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the neuro-protective effect of Xuesaitong Injection (XST) on brain inflammatory response after transient focal cerebral ischemia/reperfusion in rats.
METHODSFocal cerebral ischemia/reperfusion models of male rats were induced by transient occlusion for 2 h of middle cerebral artery (MCA) which was followed by 24 h reperfusion. XST was administered through intraperitoneal injection of 25 mg/kg or 50 mg/kg at 4 h after the onset of ischemia. After reperfusion for 24 h, the neurological function score was evaluated, the brain edema was detected with dry-wet weight method, the myeloperoxidase (MPO) activity and the expression of intercellular adhesion molecule-1 (ICAM-1) of ischemic cerebral cortex and caudate putamen was determined by spectrophotometry and immunohistochemistry respectively.
RESULTSXST not only lowered neurological function score at the dose of 50 mg/kg, but reduced brain edema and inhibited MPO activity and ICAM-1 expression as compared with the ischemia/reperfusion model group (P < 0.01).
CONCLUSIONXST has a definite effect on inhibiting the expression of ICAM-1 and neutrophil infiltration in rats with cerebral ischemia/reperfusion when treatment started at 4 h after ischemia onset, and also attenuates inflammation in the infarcted cerebral area.
