Role of PI3K/Akt signaling in hydrogen sulfide-induced alteration in expression of collagen I and III in hepatic stellate cells.
- VernacularTitle:PI3K/Akt信号转导通路在硫化氢影响肝星状细胞Ⅰ和Ⅲ型胶原表达中的作用
- Author:
Hui WANG
1
;
Qiang REN
;
Weigang CHEN
;
Rui LI
;
Lixiu SONG
;
Ning ZHANG
;
Cuihua QI
;
Yong ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Collagen Type I; metabolism; Collagen Type III; metabolism; Hepatic Stellate Cells; drug effects; metabolism; Hydrogen Sulfide; pharmacology; Phosphatidylinositol 3-Kinases; metabolism; Proto-Oncogene Proteins c-akt; metabolism; RNA, Messenger; genetics; Rats; Signal Transduction
- From: Chinese Journal of Hepatology 2014;22(6):430-433
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of the PI3K/Akt signaling pathway in hydrogen sulfide-induced alterations in expression of collagen I and collagen III in hepatic stellate cells.
METHODSIn vitro cultured rat hepatic stellate cells (HSC-T6) were treated with hydrogen sulfide, or left untreated for use as controls, and divided into groups for treatment with different inhibitors for the various factors involved in the PI3K/Akt signaling pathway. Reverse transcription-PCR was used to measure Collagen I and collagen III mRNA expression. Western blotting was used to detect the protein expression of PI3K and p-Akt, which are upstream proteins of the PI3K/Akt pathway.
RESULTSCompared with the untreated control cells, the hydrogen sulfide treated cells showed elevated expression of collagen I mRNA (F =14.635, P less than 0.05), collagen III mRNA (F =14.620, P less than 0.05), PI3K protein (F =26.672, P less than 0.05), and p-Akt (F =23.522, P less than 0.05). Compared to the cells treated with hydrogen sulfide alone, the cells treated with the various inhibitors showed lower expression of collagen I mRNA (F =14.635, P less than 0.05), collagen III mRNA (F=14.620, P less than 0.05), PI3K protein (F =26.672, P less than 0.05), and p-Akt protein (F =23.522, P less than 0.05).
CONCLUSIONHydrogen sulfide can activate the PI3K/Akt pathway and elevate the expression of collagen I and collagen III in rat hepatic stellate cells.
