Influence of PP2A activator on proliferation of HL-60 cells and analysis of PP2A activity changes in patients with acute myeloid leukemia.
- Author:
Yan YANG
1
;
Xiao-Qing LI
;
Qing HUANG
;
Shi-Ang HUANG
Author Information
1. Center for Stem Cell Research and Application, Tongji Medical College Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Apoptosis;
Case-Control Studies;
Cell Proliferation;
drug effects;
Enzyme Activators;
pharmacology;
Enzyme Inhibitors;
pharmacology;
Female;
Fingolimod Hydrochloride;
HL-60 Cells;
Humans;
Leukemia, Myeloid, Acute;
metabolism;
Male;
Middle Aged;
Okadaic Acid;
pharmacology;
Propylene Glycols;
pharmacology;
Protein Phosphatase 2;
antagonists & inhibitors;
metabolism;
Sphingosine;
analogs & derivatives;
pharmacology;
Young Adult
- From:
Journal of Experimental Hematology
2011;19(3):594-597
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the effect of PP2A activator and PP2A inhibitor on proliferation of HL-60 cells and analyze the changes of PP2A activity in patients with acute myeloid leukemia (AML), HL-60 cells were treated with FTY720 alone or in combination with okadaic acid (OA) for 24 hours in culture. Cell proliferation was assayed with CCK8 kit. In addition, 20 AML patients including de novo AML and relapsed AML were enrolled in this study. The activity of PP2A in the peripheral blood mononuclear cells of patients was assayed with a PP2A Immunoprecipitation Phosphatase Assay Kit, the data were analyzed by software SPSS 16.0. The results indicated that as compared with control group, the proliferation of cells in FTY720 group was obviously inhibited (p < 0.05). The proliferation of cells in FTY720 + OA group was slightly inhibited as compared with the control group, there was no statistical difference (p > 0.05), but there was significant difference between the FTY720 + OA and FTY720 groups (p < 0.05). The activity of PP2A in AML patients (453.67 ± 102.52 pmol phosphate) was obviously lower than that in the normal controls (673.29 ± 96.32 pmol phosphate), there was significant difference between them (p < 0.01). It is concluded that the activation or inhibition of PP2A can affect the proliferation of HL-60 cells in vitro. Compared with healthy individuals, the activity of PP2A in AML patients is obviously lower. PP2A protein playing a key role in the occurrence and development of AML may be valuable for the diagnosis and treatment of AML.
