Reversal of multidrug resistance in HL-60, MSC and CD34(+) cells from umbilical cord blood by sustained intracellular acidification.
- Author:
Ruo-Jun WANG
1
;
Guo-Qiang CHANG
;
Qing-Hua LI
;
Li-Hong WANG
;
Wei-Na JIN
;
Ya-Ni LIN
;
Hua-Wen LI
;
Tian-Xiang PANG
Author Information
1. State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
- Publication Type:Journal Article
- MeSH:
ATP-Binding Cassette, Sub-Family B, Member 1;
metabolism;
Antigens, CD34;
metabolism;
Bone Marrow Cells;
cytology;
metabolism;
Cell Physiological Phenomena;
Drug Resistance, Multiple;
Drug Resistance, Neoplasm;
Fetal Blood;
cytology;
metabolism;
HL-60 Cells;
Humans;
Mesenchymal Stromal Cells;
cytology;
metabolism
- From:
Journal of Experimental Hematology
2011;19(3):666-670
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the effect of intracellular acidification on accumulation of rhodamine 123 (rh123) in non-mature cells with none or low expression of multidrug resistance MDR1. The expression of MDR1 mRNA was detected by real-time quantitative RT-PCR. Confocal laser microscopy was used to determine the calibration curve of intracellular acidification (pHi). MTT assay was used to detect the cytotoxicity of intracellular acidification on HL-60, MSC and CD34(+) cells from umbilical cord blood. Flow cytometry was applied to measure the influence of intracellular acidification. The results indicated that the intracellular acidification had no obvious cytotoxicity on HL-60, MSC and CD34(+) cells. The acidification resulted in the increased rhodamine 123 accumulation in HL-60, MSC and CD34(+) cells without P-gp activity. Moreover, the more primitive cells, the less accumulation of intracellular Rh123 were observed. It is concluded that the intracellular acidification can reverse the MDR of HL-60, MSC and CD34(+) cells.
