Expression of radioresistant genes survivin and HO-1 in mesenchymal stem cells.
- Author:
Bing WU
1
;
Yan-Chun WANG
;
Yan WEI
;
Ya-Li ZHOU
;
Ling ZHANG
;
Qian ZHANG
;
Cun-Bang WANG
;
Hai BAI
Author Information
1. Institute of Hematological Diseases, Lanzhou Military Area Lamzhou General Hospital, Lanzhou 730050, Gansu Province, China.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
metabolism;
radiation effects;
Cell Differentiation;
Cells, Cultured;
Flow Cytometry;
Gene Expression;
Heme Oxygenase-1;
genetics;
Humans;
Inhibitor of Apoptosis Proteins;
genetics;
Mesenchymal Stromal Cells;
metabolism;
radiation effects
- From:
Journal of Experimental Hematology
2011;19(3):805-808
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the expression of radioresistant genes survivin and HO-1 in mesenchymal stem cells (MSC). Human bone marrow MSC were isolated and enriched by Fircoll density gradient centrifugation, then identified by flow cytometry. MSC were induced with dexamethasone, insulin, 3-isobutyl-1-methyl-xanthine (IBMX) and indomethacin to differentiate into adipocytes. Then the expression of survivin and HO-1 in MSC was detected by RT-PCR. The results indicated that the expressions of surface antigen CD34 and HLA-DR in MSC in vitro were negative while the expressions of CD44 and CD71 were positive. MSC could be differentiated into adipocytes by inductor. RT-PCR showed the expression of radioresistant genes survivin and HO-1 in MSC. It is concluded that MSC have lower sensitivity to radiation, which may associate with the expression of radioresistant genes survivin and HO-1 in MSC.
