Effects of Clostridium difficile toxin a on proliferation of K562 cells.
- Author:
Ming LI
1
;
Ya-Ming XI
;
Che CHEN
;
Hui-Yuan CHU
;
Hao ZHANG
;
Pei LI
;
Wei DENG
Author Information
1. Department of Hematology, Lanzhou University First Hospital, Lanzhou , Gansu Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Bacterial Toxins;
pharmacology;
Cell Proliferation;
drug effects;
Enterotoxins;
pharmacology;
Humans;
K562 Cells
- From:
Journal of Experimental Hematology
2011;19(4):894-897
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of clostridium difficile toxin A (Tcd A) on proliferation of K562 cells and its mechanism. The proliferative activity of K562 cells exposed to Tcd A was tested by MTT assay; cell cycle distribution and mitochondrial membrane potential were analyzed by flow cytometry; the protein expression of cytochrome C and DNA fragmentation were observed by immunohistochemistry staining and agarose gel electrophoresis respectively. The results indicated that Tcd A inhibited proliferation of K562 cells in a time-and concentration-dependent manner. Cells were arrested at G(0)/G(1) phase. Peak of apoptosis appeared. The protein expression of cytochrome C increased as compared with control group (p < 0.05). Agarose gel electrophoresis of DNA from K562 treated with Tcd A revealed a "ladder" pattern. It is concluded that clostridium difficile toxin A can inhibit proliferation and induce apoptosis of K562 cells. The mechanism may be in relation to decrease of mitochondrial membrane potential and the release of cytochrome C from mitochondria matrix.
