Cyclin D1, hTERT expression and telomerase activity in HL-60 and HL-60A cell lines and their significance.
- Author:
Ke-Zhi HUANG
1
;
Da-Nian NIE
;
Song-Mei YIN
;
Yi-Qing LI
;
Shuang-Feng XIE
;
Li-Ping MA
;
Xiu-Ju WANG
;
Yu-Dan WU
Author Information
1. Department of Hematology, Sun Yat-Sen University, Guangzhou, Guangdong Province, China.
- Publication Type:Journal Article
- MeSH:
Cell Cycle;
Cyclin D1;
metabolism;
HL-60 Cells;
Humans;
Leukemia;
metabolism;
Telomerase;
metabolism
- From:
Journal of Experimental Hematology
2011;19(4):911-915
- CountryChina
- Language:Chinese
-
Abstract:
To observe the expression of cyclin D1, hTERT, and telomerase activity in MNC, HL-60, HL-60A and to explore their effects on leukemogenesis and drug-resistance, normal human peripheral blood mononuclear cells, HL-60 cells sensitive to adriamycin and HL-60A cells resistant to adriamycin were investigated. The cell cycle was analyzed by flow cytometry, and the apoptosis was analyzed by Annexin V-FITC(+) PI staining. Expressions of cyclin D1 and hTERT were determined by real-time PCR and Western blot. Telomerase activity was detected by TRAP-ELISA. The results indicated that the percentage of MNC, HL-60 and HL-60A in S phase was (10.21 + 2.11)%, (44.93 + 3.00)%, and (51.38 + 1.10)% respectively; the percentage of apoptosis cells was (16.14 + 2.13)%, (7.53 + 0.92)%, (4.15 + 0.96)% respectively; the expression of mRNA and protein for cyclin D1 and hTERT increased; the telomerase activities of HL-60 and HL-60A were higher (p = 0.000), whereas the difference between HL-60 and HL-60A was no statistically significant (p = 0.232); positive correlation between cyclin D1, hTERT and telomerase activity had been found (p < 0.01). It is concluded that the cells of S phase increased while the apoptotic cells decreased in HL-60 and HL-60A, especially in HL-60A, which may be due to the up-regulation of cyclin D1, hTERT and telomerase activity.
