Effects of decitabine on biological behavior of U266 cells.
- Author:
Mei-Fang WANG
1
;
Lin-Hua YANG
;
Chun-Xia DONG
;
Rui-Juan ZHANG
;
Jian-Hua ZHANG
;
Zhi-Ping GUO
;
Jian-Fang CHEN
;
Li ZHAGN
;
Da-Wei FENG
Author Information
1. Department of Hematology, Shanxi Medical University Second Hospital, Taiyuan 030001, Shanxi Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Azacitidine;
analogs & derivatives;
pharmacology;
Cell Cycle;
drug effects;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Humans;
Multiple Myeloma;
pathology
- From:
Journal of Experimental Hematology
2011;19(4):944-948
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore the effects of decitabine on the biological behaviour of U266 cells in vitro so as to provide a new thinking and experiment basis, as well as new evidences for the pathogenesis of multiple myeloma. MTT and colony formation assays were used to evaluate the impact of decitabine on the ability of proliferation of U266 cells; flow cytometry was used to analyze the cell distribution in cell cycle; transwell chamber and matrigel assays were used to observe the ability of migration and invasion. The results indicated that decitabine could significantly suppress the proliferation of U266 cells in time-and dose-dependent manners. The flow cytometric analysis demonstrated that the cells in G(0)-G(1) phase significantly increased while the cells in S and G(2)/M phase decreased. The migration and matrigel invading tests showed that the number of cells moving into under chamber of transwell decreased after U266 cells treated with decitabine. It is concluded that decitabine may act as an effective drug for MM by inhibiting the proliferation, migration and invasion ability, and the specific mechanism needs to be deeply explored.
