Effect of Bushen Qianggu decoction on the proliferation of synovial fibroblasts and expression of PCNA and Bcl-2.
- Author:
Hua-Li GAO
1
;
Gui-Lin OUYANG
;
Xin-Xing HUANG
;
Ning-Li LI
;
Lian-Bo XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Cell Proliferation; drug effects; Drugs, Chinese Herbal; pharmacology; Female; Fibroblasts; drug effects; physiology; Proliferating Cell Nuclear Antigen; analysis; Proto-Oncogene Proteins c-bcl-2; analysis; Rats; Rats, Wistar; Synovial Membrane; chemistry; cytology; drug effects
- From: China Journal of Orthopaedics and Traumatology 2012;25(11):942-945
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of Bushen Qianggu decoction proliferation and PCNA and Bcl-2 expression.
METHODSSerum containing BQD was made and synovial fibroblasts were separated and cultured and passaged in vitro. Four groups were divided as 20% blank control group, serum containing 20% Tripterygium wilfordii multi-glycosides drug (TWMD), 20% of serum containing high and low of BQD, respectively. Serum containing drugs of different concentration were added into the synovial fibroblasts of the third generation, and then the synovial fibroblasts were cultured continued. The effects of different drugs on synovial fibroblasts and PCNA and Bcl-2 expression were observed.
RESULTSCompared with the control serum, BQD-containing serum promoted the apoptosis of synovial fibroblasts (P < 0.000 1); especially, high dose could inhibit proliferation. The expression of PCNA and Bcl-2 was significantly lower in BQD-containing serum (P < 0.000 1 vs control group).
CONCLUSIONBQD can promote the apoptosis of synovial fibroblasts by improving of expression of PCNA and Bcl-2, which may be one of the mechanisms of BQD in preventing and treating osteoporosis of rheumatoid arthritis.
