Preparation and evaluation of microbubble ultrasound contrast agent with N-carboxymethyl chitosan.
- Author:
Feng LÜ
1
;
Su-Xia CHEN
;
Tian-Jun LIU
;
Hong-Fan SUN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chitin; analogs & derivatives; chemical synthesis; chemistry; Contrast Media; Liver; diagnostic imaging; Microbubbles; Rabbits; Ultrasonics; Ultrasonography
- From: Acta Academiae Medicinae Sinicae 2006;28(4):497-501
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare microbubble, made of N-carboxymethyl chitosan, as ultrasound contrast agent and evaluate its characteristics and acoustic effects in vivo.
METHODSOil-Water-Oil multiple emulsion/solvent evaporation method was used to prepare the microbubble contrast agent. Both optical micrography and scanning electron micrography were performed to determine the bubble size and morphology. The acoustic effect of the N-carboxymethyl chitosan echo contrast agent was evaluated in vivo in rabbit. Liver echo images were recorded with ultrasound machine before and after intravenous bolus injecting 0.5 ml of the agent.
RESULTSThe novel N-carboxymethyl chitosan echo contrast agent was formulated as lyophilized product, with a mean diameter of 2-3 microm and a shell thickness of 250-300 nm. Its size is relatively uniform. The imaging effect was remarkably enhanced with the ultrasonic contrast agent when applied in rabbit livers.
CONCLUSIONIt is feasible to prepare excellent microbubble ultrasound contrast agent with N-carboxymethyl chitosan as membrane components.
