Resistance to HIV-1 infection of CD4 + T lymphocytes in vitro from chinese HIV-1 exposed seronegative individuals.
- Author:
Yan-li SU
1
;
Hong SHANG
;
Jing LIU
;
Shu-xiang WANG
;
Wen-qing GENG
;
Hua-lu CUI
;
Yong-jun JIANG
;
Ya-nan WANG
;
Zi-ning ZHANG
;
Ya-ting WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; CD4-Positive T-Lymphocytes; immunology; virology; China; Female; HIV; classification; isolation & purification; pathogenicity; HIV Infections; virology; HIV Seronegativity; immunology; Humans; In Vitro Techniques; Male; Sexual Partners
- From: Acta Academiae Medicinae Sinicae 2006;28(5):613-617
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the relative resistance to HIV-1 infection of CD4 + T lymphocytes in HIV-exposed seronegative individuals (ESNs) in China.
METHODSHIV primary isolates were obtained from peripheral whole blood of HIV-infected persons. CD4 + T lymphocytes of Chinese ESNs were separated from peripheral blood mononuclear cells with magnetic cell sorting (MACS). The purified CD4 + T lymphocytes were cocultured with HIV primary isolates. The p24 level was detected and the culture medium was refreshed every 3 days within 2 weeks.
RESULTSFor M tropic HIV strains, p24 level was significantly lower in ESN group than in control group (P < 0.05); for some M tropic HIV strains, even no p24 replicated in ESN group. However, T tropic virus strains had no significant difference between these two groups (P > 0.05).
CONCLUSIONCD4 + T lymphocytes of Chinese ESNs may possess relative resistance to M tropic HIV strains, which may be one of the main influencing factors that result in ESN.
