Background study of HIV-1 drug resistant mutations in treatment-naive patients in liaoning province.
- Author:
Xiao-xu HAN
1
;
Min ZHANG
;
Di DAI
;
Chunming LU
;
Zi-ning ZHANG
;
Jing LIU
;
Ya-nan WANG
;
Yong-jun JIANG
;
Hong SHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; China; epidemiology; Drug Resistance, Viral; genetics; Female; HIV Infections; drug therapy; epidemiology; HIV Protease; genetics; HIV Reverse Transcriptase; genetics; HIV-1; genetics; Humans; Male; Molecular Epidemiology; Mutation; Sequence Analysis, DNA
- From: Acta Academiae Medicinae Sinicae 2006;28(5):632-636
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo collect background information on drug resistance mutations in treatment-naïve HIV-1 infected individuals in Liaoning Province.
METHODSSamples from 91 antiretroviral therapy-naïve patients were collected. The entire protease gene and 1-290 amino acids of the reverse transcriptase gene were amplified by nested PCR from provirus DNA and sequenced. The results were analyzed with HIVdb-Drug Resistance Algorithm, and genotypic resistance mutations were determined to particular anti-HIV drugs.
RESULTSTotally 91 sequences were obtained, 3 of which displayed M46I mutations in the protease gene. Minor resistance mutation rate to protease inhibitors was 100%, including types of L63P (60.4%), V77I (60.4%), M36I/V (31.9%), A71V/T (22.0%), L10I (8.8%), and K20R (6.6%). Only one sequence carried reverse transcriptase related resistance mutations M184I.
CONCLUSIONSAbout 4.4% of HIV-1 infected individuals in Liaoning Province carried strains with drug resistance mutations. Most treatment-naïve HIV-1 infected individuals in Liaoning Province were sensitive to the currently available antiviral medicines, but antiviral treatment must be in accordance with the strict procedures to keep better adherence and avoid the prevalence of drug-resistant strains.
