Effects of butyrylchitosan on expression of proliferating cell nuclear antigen in fibroblast proliferation of rabbit eyes after filtering operation.
- Author:
Ping WEI
1
;
Ai-qin WANG
;
Xun-wen LEI
;
Zhi-xin CAO
;
Xiao-lin LI
;
Qin WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; drug effects; Chitosan; analogs & derivatives; pharmacology; Female; Fibroblasts; cytology; metabolism; Filtering Surgery; Male; Membranes, Artificial; Proliferating Cell Nuclear Antigen; biosynthesis; Rabbits
- From: Acta Academiae Medicinae Sinicae 2006;28(6):813-816
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of butyrylchitosan on the expression of proliferating cell nuclear antigen ( PCNA) in fibroblast proliferation of rabbit eyes after filtering operation.
METHODSTwenty-four New Zealand rabbits were randomly divided into 2 groups, with 12 rabbits in each group. Rabbits in one group received butyrylchitosan under scleral patch of trabeculectomy in right eyes and trabeculectomy in left eyes (trabeculectomy group). Rabbits in the other group received mitomycin C (MMC) in trabeculectomy in right eyes (MMC group) and without operation in left eyes. Rabbits were killed 1, 4, and 12 weeks after operations. Immunohistochemical staining was used to detected PCNA expression in fibroblast.
RESULTSAfter use of butyrylchitosan, the PCNA expression significantly decreased compared with trabeculectomy group (P < 0. 001). PCNA expression in MMC group was significantly lower than in trabeculectomy group (P <0. 001).
CONCLUSIONUsing butyrylchitosan under scleral patch of trabeculectomy decreases PCNA expression in proliferating cell and inhibits the scarring at filtering site.
