Expression of hypoxia inducible factor-1α and correlated target genes in human laryngeal carcinoma.
- Author:
Ou XU
1
;
Xiao-ming LI
;
Mao-xin WANG
;
Yong-tao QU
;
Xiu-ying LU
;
Chun-guang SHAN
;
Qing-jia SUN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Carcinoma, Squamous Cell; metabolism; pathology; Cell Line, Tumor; Female; Glucose Transporter Type 1; metabolism; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; metabolism; Laryngeal Neoplasms; metabolism; pathology; Male; Middle Aged; Vascular Endothelial Growth Factor A; metabolism
- From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2012;47(4):330-333
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the expression of hypoxia inducible factor 1 alpha (HIF-1α), glucose transporter protein-1 (GLUT-1) and vascular endothelial growth factor (VEGF) in human laryngeal carcinoma tissue, and to study the relationship between hypoxia and HIF-1α, GLUT-1, VEGF in human laryngeal carcinoma Hep-2 cells and to explore the effect of HIF-1α, GLUT-1 and VEGF as endogenous hypoxic markers on laryngeal carcinoma.
METHODSThe expression levels of HIF-1α, GLUT-1 and VEGF were detected in 35 cases of laryngeal carcinoma by SP immunohistochemical methods and in Hep-2 cells by SP immunocytochemical methods. The relationship between HIF-1α and GLUT-1, VEGF protein expression was analyzed.
RESULTSOf the 35 cases, 16 cases expressed HIF-1α, 16 cases expressed GLUT-1, 19 cases expressed VEGF. The expression of HIF-1α and VEGF were closely correlated with pathologic grading and lymphnode metastasis. GLUT-1 was correlated with lymphnode metastasis. The expression levels of HIF-1α, GLUT-1 and VEGF in Hep-2 cells under hypoxic condition were higher than those under normoxic condition.
CONCLUSIONHIF-1α may promote the expression of GLUT-1 and VEGF in laryngeal carcinoma, furthermore promote tumor angiogenesis, invasion, and metastasis of the laryngeal carcinoma.
